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| ªí 1.¡@°ò¦¹}³²Õ¦¨ Table 1. The composition of basal diet |
||
| ¦¨ ¤À | Ingredients | §t ¶q amount, % |
| ¶À ¥É ¦Ì ¤j ¨§ ²Ë ¥É¦Ì¾¢½è¯» ¤j ¨§ ªo ²Ê ÁR ¥Û¦Ç¥Û¯» ÁC»Ä²B¶t ºû¥Í¯À¡ÐÄqª«½è¹w²Vª«a ¸K ¤Æ ÆQ ´â¤ÆÁxú^ ¼ÚùªÎ SP-500 DL¡Ð¥Ò²¸Ói»Ä ¡@ ¦¨¤À¦ôºâÈ ²Ê³J¥Õ½è ¥N Á ¯à ¥Ò²¸Ói»Ä Â÷ Ói »Ä |
Yellow corn, ground Soybean meal (43.5%) Corn gluten meal (60%) Soybean oil Rice hull Limestone, pulverized Dicalcium phosphate Vitamin-mineral premixa Iodized salt Choline chloride, 50% Aurafac SP-500 DL-Methionine ¡@ Calculated value Crude protein, % Metabolizable energy, kcal/kg Methionine, % Lysine, % |
61.0 18.0 12.0 1.0 3.5 1.3 2.0 0.5 0.3 0.1 0.1 0.2 ¡@ ¡@ 20.0 2980 0.67 0.80 |
| a ¨C¤½¤ç¹}³ºû¥Í¯À¡ÐÄqª«½è²K¥[¶q¡GVitamins and minerals added per kilogram of diet: Vit. A, 4,500 IU; Vit. D3, 900 ICU; Vit. E, 12 IU; Vit. K3, 1 mg; Vit. B1, 2 mg; Vit. B2, 4 mg; Vit. B6, 4.5 mg; Vit. B12, 0.006 mg; Biotin, 0.2 mg; Folic acid, 1 mg; Niacin, 70 mg; Calcium pantothenate, 11 mg; FeSO4.7H2O, 0.4 gm; CuSO4.5H2O, 0.031 gm; MnSO4.5H2O, 0.26 gm; ZnO, 0.075 gm; Na2SeO3, 0.4 mg; KI, 0.6 mg. | ||
¡@
| ªí 2.¡@¹}®Æì®Æ¤§Ói°ò»Ä²Õ¦¨ Table 2. The composition of amino acids in feed ingredients |
|||
| Ói°ò»Ä Amino acids ¢H | ¹}®Æì®Æ Feed ingredients | ||
| ¤j¨§²Ë Soybean meal | ¥É¦Ì¯» Corn meal | ¥É¦Ì¾¢½è¯» Corn gluten meal | |
| ¤Ñªù¥V»Ä Aspartic acid ßm¤BÓi»Ä Threonine µ· Ói »Ä Serine ²ã Ói »Ä Proline ¥Ì Ói »Ä Glycine ¤þ Ói »Ä Alanine õé Ói »Ä Valine ¥Ò²¸Ói»Ä Methionine ²§¥ÕÓi»Ä Isoleucine ¥Õ Ói »Ä Leucine ¹T Ói »Ä Tyrosine f¤þÓi»Ä Phenylalanine ²Õ Ói »Ä Histidine Â÷ Ói »Ä Lysine ºë Ói »Ä Arginine ¡@ ²Ê³J¥Õ½è Crude protein |
5.55 1.66 1.78 2.43 1.79 1.87 2.19 0.82 2.54 3.85 2.12 2.86 1.32 3.11 3.61 ¡@ 43.60 |
0.57 0.29 0.35 0.71 0.28 0.55 0.61 0.33 0.50 1.20 0.57 0.51 0.25 0.27 0.03 ¡@ 8.30 |
4.43 2.12 2.78 6.75 1.73 5.88 2.85 1.73 2.52 13.14 4.54 5.45 1.53 1.30 2.36 ¡@ 59.40 |
¸ÕÅç©Ò´ú±o¤§¼Æ¾Ú¥ý¶i¦æÅܤè¤ÀªR«á¡A¦A¥H¾HªÖ¤ó·s¦hÅܰìªk¡]Duncan's new multiple range test; Steel and Torrie, 1980¡^¤ñ¸û¤£¦P³B²z¶¡ªº®t²§ÅãµÛ©Ê¡C¥H¸ÕÅç 2 ¤§ÂúÃZ¦b 0¡ã2 ¶gÄ֤Π0¡ã4 ¶gÄÖ¤§¼W«¡A¹ï¹}³¤¤Â÷Ói»Ä§t¶q¶i¦æ°jÂk¤ÀªR¡AµM«á¦A¥H¦±´Òªk¡]Bent-stick method; Morris, 1983¡^¡A¨D¥XÂúÃZ¦b 0¡ã2 ¶gÄ֤Π0¡ã4 ¶gÄÖ¤§Â÷Ói»Ä»Ýn¶q¡C
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¸ÕÅç 2 ÂX¤jÂ÷Ói»Äªº²K¥[¯Å¶Z¬°¨C¯Å 0.2%¡A±q 0.8% ¤@ª½´£°ª¨ì 1.6%¡Cµ²ªGÅã¥Ü¡A¦b 0¡ã2 ¶gÄ֮ɹ}³¤¤§tÂ÷Ói»Ä 1.2% ¥H¤W®É¡AÂúÃZªº¼W«¤Î¹}®Æ§Q¥Î®Ä²v§Y¨S¦³®t²§¡]P¡Ö0.05¡^¡F¦Ó¹}³§tÂ÷Ói»Ä 1.4% ªÌ¥Íªø©Ê¯à¦³¸û¨ÎªºÁͶաA¦ýÂ÷Ói»Ä§t¶q°ª©ó 1.4% «á¹ï¥Íªø©Ê¯à¤Ï¦Ó¦³¤£§Qªº¼vÅT¡C¦b 0¡ã4 ¶gÄÖÂúÃZªº¹}³¤¤§tÂ÷Ói»Ä 1.0% ¥H¤WªÌ¡A¼W«¤Î¹}®Æ§Q¥Î®Ä²v§¡µL®t²§¡]P¡Ö0.05¡^¡F¦Ó¹}³§tÂ÷Ói»Ä 1.2% ªÌ¥Íªø©Ê¯à¦³¸û¨ÎªºÁͶաC¹}³Â÷Ói»Ä§t¶q¶W¹L 1.2% ªÌ¡A¹ï¥Íªø©Ê¯à¤Ï¦Ó¦³¤£§Qªº¼vÅT¡C
¥H 0¡ã2 ¶gÄ֤Π0¡ã4 ¶gÄÖÂúÃZªº¼W«È¹ï¹}³¤¤Â÷Ói»Ä§t¶q¶i¦æ°jÂk¤ÀªR¡A¦A¥H¦±´Òªk¨D¥X 0¡ã2 ¶gÄÖÂúÃZªºÂ÷Ói»Ä»Ýn¶q¬° 1.36%¡]¹Ï 1¡^¡F0¡ã4 ¶gÄÖÂúÃZªºÂ÷Ói»Ä»Ýn¶q¬° 1.16%¡]¹Ï 2¡^¡C
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| ªí 3.
¹}³Â÷Ói»Ä§t¶q¹ï 0¡ã4 ¶gÄÖÂúÃZ¥Íªø©Ê¯àªº¼vÅT Table 3. Effect of dietary lysine levels on growth performance of goslings (0-4 wk) |
|||
| Â÷Ói»Ä Lysine ¢H | ±Ä¹¶q Feed intake g/bird/4 wk | ¼W« Gain g/4 wk | ¹}®Æ/¼W« Feed/Gain |
| ¸Õ Åç 1. Trial 1. 0-2 wk of age 0.8 0.9 1.0 1.1 1.2 0-4 wk of age 0.8 0.9 1.0 1.1 1.2 ¸Õ Åç 2. Trial 2. 0-2 wk of age 0.8 1.0 1.2 1.4 1.6 0-4 wk of age 0.8 1.0 1.2 1.4 1.6 ¸Õ Åç 3. Trial 3. 5-19 day of age 1.1 1.2 1.3 1.4 1.5 5-33 day of age 1.1 1.2 1.3 1.4 1.5 |
¡@ ¡@ 900 930 950 970 980 ¡@ 3736 3837 3956 4104 3926 ¡@ ¡@ 750 830 860 870 930 ¡@ 2851 3422 3465 3558 3340 ¡@ ¡@ 1284 1255 1451 1430 1446 ¡@ 3859 4129 4132 4036 3950 |
¡@ ¡@ 572a 591ab 638bc 644bc 660c ¡@ 1974a 2043ab 2131bc 2131bc 2195c ¡@ ¡@ 401a 498b 550c 579c 559c ¡@ 1552a 1906b 2014b 1957b 1936b ¡@ ¡@ 842 835 862 875 897 ¡@ 2005a 1973ab 1979ab 1809b 1872b |
¡@ ¡@ 1.64ab 1.66a 1.48c 1.53bc 1.52bc ¡@ 1.97 1.96 1.90 1.96 1.85 ¡@ ¡@ 1.89a 1.68ab 1.53b 1.50b 1.66ab ¡@ 1.84 1.80 1.77 1.82 1.79 ¡@ ¡@ 1.63 1.54 1.70 1.63 1.62 ¡@ 1.93 2.13 2.09 2.22 2.11 |
| a,b,c Data with different superscripts within the same column differ significantly (P¡Õ0.05). | |||

¸ÕÅ礧µ²ªGÅã¥ÜÂúÃZªº¥Íªø³t²v¼W§Ö®É¡AÀç¾i¤Àªº»Ýn¶q¤]ÀH¤§´£°ª¡CMorris and Abebe¡]1990¡^¤Î Scott et al.¡]1982¡^¦bÂû°¦ªº¸ÕÅç¤]»{¬°¡AÂû°¦±ý¹F³Ì¤j¥Íªø³t²v©Ò»Ý¤§Â÷Ói»Ä¡A·|ÀHµÛ¹}³¤¤³J¥Õ½è§t¶q¤§¼W¥[¦Ó´£°ª¡CBlum¡]1987¡^¤Î Nitsan et al.¡]1983¡^©Ò±ÀÂËÃZ°¦Â÷Ói»Ä»Ýn¶q¡A¤À§O¬° 0.95% ¤Î 1.07% ¬O°ò©ó²Ê³J¥Õ½è¤À§O¬° 17% ¤Î 18.8% ¤Î¥NÁ¯à¤À§O¬° 2800 kcal/kg ¤Î 2987 kcal/kg ®Éªº±ÀÂ˶q¡CSummer et al.¡]1987¡^¥H§t²Ê³J¥Õ½è 16% ªº¹}³Áýµ¹·R¨dµnÃZ®É¡A±ÀÂ˹}³¤¤»Ý§t 0.81% Â÷Ói»Ä¡CChen and Shen¡]1979¡^¥H§t²Ê³J¥Õ½è 18% ¤§¹}³Áýµ¹Âú¤gµfÀn®É¡A¹}³¤¤Á`Â÷Ói»Äªº§t¶q¬°1.06%¡C¥»¸ÕÅç¥H²Ê³J¥Õ½è 20%¡B¥NÁ¯à 2980 kcal/kg¡A¦b 0¡ã2 ¶gÄ֤Π5¡ã19 ¤éÄÖªº¥Íªø©Ê¯àªí²{Åã¥Ü¡A¹}³¤¤§tÂ÷Ói»Ä 1.4% ©Î 1.5% ®É¡A¼W«¦³¸û¦nªºÁͶաFÂ÷Ói»Ä§t¶q¦A´£°ª®É¡A«h¦³¥Íªø§í¨îªº²{¶H¡C¦b 0¡ã4 ¶gÄ֮ɡA¥H¹}³¤¤§tÂ÷Ói»Ä 1.2% ªÌ¼W«¸û¦h¡A¦ý¹}³¤¤Â÷Ói»Ä¦A´£°ª¦Ü 1.4% ©Î¥H¤W®É¡A«hÅã¥Ü¤F§í¨îªº²{¶H¡CJones¡]1961¡^¤Î Jones¡]1964¡^¦b¥ÍªøÂû¹}³²K¥[¹L¶qÂ÷Ói»Ä¡]2% L¡ÐÂ÷Ói»Ä¡^¡A¤]¦³¥Íªø¿ðº¢ªº²{¶H¡C³¯¡]1977¡^¦bº¡¨¬Âú¤gµfÀn»Ýn¶q¡]1.01%¡^ªº¹}³¤¤¡AÃB¥~²K¥[ 0.5% Â÷Ói»Ä®É¡A¹ï¤gµfÀn¥Íªø¤]¦³»´·L§í¨î§@¥Î¡C¥»¸ÕÅçÅã¥ÜÂúÃZ¹}³¤¤§tÂ÷Ói»Ä 0.8% ®É¼W«¸û®t¡A²K¥[¦X¦¨Â÷Ói»Ä 0.2% «á¡A¥Íªø©Ê¯à§Y¦³ÅãµÛ§ïµ½¡CSugahara and Kubo¡]1992¡^¥HÂ÷Ói»Ä¤£¨¬ªº¹}³¡]5.95 g/kg¡^Áýµ¹¥ÍªøÂû¡A¨ä¥Íªø©Ê¯à¤]ÅãµÛ¸û®t¡A¦ý²K¥[Â÷Ói»Ä«á¡A¥ç¦³ÅãµÛ§ï¶i¡C
| ªí 4.
¹}³Â÷Ói»Ä§t¶q¹ï 0¡ã4 ¶gÄÖÂúÃZ¦å²M¤¤Ói°ò»Ä¤ÎÂàÓiúA¬¡©Êªº¼vÅT Table 4. The effects of dietary lysine levels on serum amino acid and aminotransferase activity of goslings (0-4 wk) |
|||||||
| Â÷ Ói »Ä Lysine % | Â÷ Ói »Ä Lysine mg/dl | ¥Ò²¸Ói»Ä Methi-onine mg/dl | f¤þÓi»Ä Phenyl-alanine mg/dl | ºë Ói »Ä Argi-nine mg/dl | ¦â Ói »Ä Trypto-phan mg/dl | ¤Ñªù¥V»ÄÂàÓiúA Aspartate aminotrans-ferase U/l | ¤þÓi»ÄÂàÓiúA Alanine aminotrans-ferase U/l |
| ¸ÕÅç 1. Trial 1 0.8 0.9 1.0 1.1 1.2 ¸ÕÅç 2. Trial 2. 0.8 1.0 1.2 1.4 1.6 ¸ÕÅç 3. Trial 3. 1.1 1.2 1.3 1.4 1.5 |
¡@ ¡Ð ¡Ð ¡Ð ¡Ð ¡Ð ¡@ 15.22a 23.20ab 27.47b 23.53ab 26.15b ¡@ ¡Ð ¡Ð ¡Ð ¡Ð ¡Ð |
¡@ ¡Ð ¡Ð ¡Ð ¡Ð ¡Ð ¡@ 10.20ab 13.02b 14.57c 9.13ab 8.52a ¡@ ¡Ð ¡Ð ¡Ð ¡Ð ¡Ð |
¡@ ¡Ð ¡Ð ¡Ð ¡Ð ¡Ð ¡@ 17.47ab 20.58ab 23.83a 15.03ab 14.32b ¡@ ¡Ð ¡Ð ¡Ð ¡Ð ¡Ð |
¡@ ¡Ð ¡Ð ¡Ð ¡Ð ¡Ð ¡@ 35.70 50.85 43.15 35.37 40.35 ¡@ ¡Ð ¡Ð ¡Ð ¡Ð ¡Ð |
¡@ ¡Ð ¡Ð ¡Ð ¡Ð ¡Ð ¡@ 25.08ab 29.95a 30.93a 23.17ab 20.82b ¡@ ¡Ð ¡Ð ¡Ð ¡Ð ¡Ð |
¡@ 42.5 38.9 42.4 32.7 38.4 ¡@ 39.3 44.3 56.1 36.2 51.9 ¡@ 47.6 48.0 43.7 31.9 37.7 |
¡@ 19.6a 15.3b 16.1b 13.6b 14.9b ¡@ 29.5a 16.3ab 16.3ab 13.6b 17.6ab ¡@ 13.6 16.8 14.8 13.4 18.7 |
| a,b,c The data with different superscripts within the same column differ significantly (P¡Õ0.05). | |||||||
·í¹}³¤¤Â÷Ói»Ä§t¶q¶W¹L»Ýn¶q®É¡A¹ï 0¡ã4 ¶gÄÖ©Î 5¡ã33 ¤éÄÖÂúÃZªº±Ä¹¶qªº¼vÅT¤£ÅãµÛ¡A¦ý¹}³¤¤Â÷Ói»Ä§t¶q¹F 1.4% ©Î¥H¤W®É¡A±Ä¹¶q¦³°§CªºÁͶաCÁýµ¹¥ÍªøÂû°¦Ói°ò»Ä¤£¥¿Åªº¹}³¡]Fisher and Shapiro, 1961¡^¡A©ÎÁýµ¹Âû°¦³æ¤@¥²»ÝÓi°ò»Ä¹L¶qªº¹}³¡]Edmond and Baker, 1987¡^¡A§¡ªí²{¥X±Ä¹¶q°§C¤Î¥Íªø©µ½wªº²{¶H¡A¦ý¥un§â¹}³¤¤Ói°ò»Ä§t¶q½Õ¾ã¨ì§¡¿Å®É¡A±Ä¹¶q§Y·|¼W¥[¡A¥Íªø¤]·|±o¨ì§ï¶i¡]Harper, 1956¡^¡C
ÂúÃZ¹}³¤¤Â÷Ói»Ä§t¶q¥Ñ 0.8% ´£°ª¨ì 1.2% ®É¡A¦å²M¤¤Â÷Ói»Ä§t¶q·|ÅãµÛªº¥Ñ 15.22 m mole/ml ´£°ª¨ì 27.47 m mol/ml¡F¦¹µ²ªG»P Zimmerman and Scott¡]1965¡^¦bÂû©Ò¶i¦æ¤§¸ÕÅçµ²ªG¦³¬Û¦PÁͶաC¦å¼ß¤¤ºëÓi»Ä§t¶qªº®t²§¤£ÅãµÛ¡A¦ý¦b¹}³¤¤§tÂ÷Ói»Ä 1.0% ®É¦³¸û°ªªºÁͶաAÂ÷Ói»Ä¦A¼W¥[®É¡A¦å¤¤ºëÓi»Ä¤Ï¦Ó¦³µy¬°°§Cªº²{¶H¡A¦¹µ²ªG»P Ohno and Tasaki¡]1972¡^¦bÂû°¦¤§¸ÕÅçµ¹¤©ÃB¥~ªºÂ÷Ói»Ä®É¡A¦³¬Û¦üªºÁͶաC
¹}³¤¤Â÷Ói»Ä§t¶q¹ïÂúÃZ¦å²M¤¤¤Ñªù¥V»ÄÂàÓiúA¬¡©Êªº¼vÅT¤£ÅãµÛ¡C½²¡]1993¡^¥H¤gµfÀnÁý¹}¤£¦PÂ÷Ói»Ä§t¶qªº¹}³®É¡A¤]¦³¬Ûªñªºµ²ªG¡C·í¹}³¤¤§t 0.8% Â÷Ói»Ä®É¡A¦å²M¤¤¤þÓi»ÄÂàÓiúAªº¬¡©Ê¡A«oÅãµÛªº°ª©ó¹}³¤¤§tÂ÷Ói»Ä°ª©ó 0.8% ¥H¤WªÌ¡C¦ý¦U³B²z²Õ¤§È§¡¦b 13.6 U/l ¦Ü 19.6 U/l ¤§¶¡¡A¤´¦b¤@¯ë®a¸V¥¿±`¥Í²zȪº¤À§G½d³ò¤º¡]Bogin, 1992¡^¡C
»x¡@ÁÂ
¥»¸ÕÅç©Ó¦æ¬F°|¹A·~©eû·|¸É§U¸ÕÅç¸g¶O¡]82 ¬ì§Þ¡Ð2.12¡Ðªª¡Ð04(9)¡^¡AÂÔ»xÁ§աC¥»¬ì§f»ï¦t³Õ¤h¨ó§U½×¤å¤§×¥¿¡A¥çªí¥Ñ°JªºÁ·N¡C
°Ñ¦Ò¤åÄm
³¯«O°ò¡C1977¡C¤gµfÀn¤§ºëÓi»Ä»PÂ÷Ói»Ä»Ýn¶q¡CºÓ¤h½×¤å¡C°ê¥ß¥xÆW¤j¾Ç¡C
®}ªü¨½¡BªL·ç½´¡C1989¡CÂú¤pÅ髬¦³¦â¦×Âû¤§§t²¸Ói°ò»Ä»Ýn¶q¡C¤¤°ê¯bªª¾Ç·|·|»x 18(2)¡G13¡ã20¡C
»OÆW¬Ù¹A·~¦~³ø¡C1995¡C»OÆW¬Ù¬F©²¹AªLÆU¡Ap. 171¡C
½²¥@¤@¡C1993¡C¹}³¤¤Â÷Ói»Ä¡B§t²¸Ói°ò»Ä§t¶q¹ï©óÂú¤gµfÀnÂàÓiúA¬¡©Ê¤§¼vÅT¡CºÓ¤h½×¤å¡C°ê¥ß¥xÆW¤j¾Ç¡C
¨H²K´I¡C1988¡CÀn°¦Àç¾i¤À»Ýn¶q¤â¥U¡C°ê¥ß»OÆW¤j¾Ç¦L¡C
¿cª÷Âí¡B®}ªü¨½¡C1989¡C¹}³«¬ºA»P¥[¤u¤è¦¡¹ïÃZ°¦¥Íªø©Ê¯à¤Î±OÅé©Êª¬ªº¼vÅT¡C¯b²£¬ã¨s¡A22(1)¡G9¡ã19¡C
¿cª÷Âí¡B®}ªü¨½¡C1994¡CÂúÃZ¹ï¯à¶q¤Î³J¥Õ½è»Ýn¶qªº´ú©w¡C¥xÆW¯bªªÃ~Âå¾Ç·|·|³ø¡A64¡G13¡ã22¡C
¿cª÷Âí¡B®}ªü¨½¡B³¯¹B¶¯¡B³¯®Ê»a¡C1992¡CÂúÃZ¹ïÁxú^¤Î¥Ò²¸Ói»Ä»Ýn¶q¡C¤¤°ê¯bªª¾Ç·|·|»x¡A21(2)¡G157¡ã168¡C
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¡@
¡@
Lysine Requirements of White Roman Goslings
Jin-Jenn Lu(1), Shyh-Renn Chou(2), Yen-Ming Hong(1),
Kuo-Chin Wu(3), Lih-Tzyy Yeh(3) and Ching-Chang Chen(4)
Received Nov. 30, 1995; Accepted Apr. 15, 1996
ABSTRACT
Five hundred and fifty sexed White Roman goslings were allotted in three trials, to study the lysine requirements of goslings from 0 to 4 weeks of age. In trial 1, the basal diet was composed of corn-soybean meal and corn gluten meal. Lysine levels were from 0.8% in the basal diet up to 1.2% by increment of 0.1% with L-lysine supplementation. Each treatment had 2 replicates of 15 goslings per replicate. In trial 2, lysine levels were from 0.8% to 1.6% with a 0.2% lysine stepwise increment. In trial 3, lysine levels were from 1.1% to 1.5% with a 0.1% increment. In trial 2 and trial 3, each diet was fed to 2 replicates of 20 goslings each in 5 treatments. Growth performance was measured and blood content of lysine and transaminases were analyzed, as the criteria of measuring the lysine requirements.
No differences in weight gain (P¡Ö0.05) among treatments were found as dietary lysine were above 1.0%. Weight gain and feed/gain ratio reached the best when the diet contained 1.2% lysine. However, when lysine was above 1.3%, inferior growth performance showed up. In trial 2, the optimum lysine requirements were estimated through regression analysis. The optimum lysine requirement for goslings with 0-2 weeks of age were estimated to be 1.36% in diet, and 1.16% in diet for 0-4 weeks of age.
The impact of diet lysine on blood characteristics of goslings was quite variable. From trial 2, lysine in serum was found low and the alanine aminotransferase was high with a 0.8% dietary lysine level. When the dietary lysine was above 1.0%, lysine in serum increased and alanine aminotransferase decreased.¡]Key Words¡GWhite Roman Goslings, Lysine, Requirements¡^
¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w¢w
(1)Department of Animal Science, National Chia-Yi
Institute of Agriculture, Chia-Yi 60083, Taiwan, Republic of China.
(2)Department of Veterinary Medicine National Chia-Yi Institute of Agriculture,
Chia-Yi 60083, Taiwan, Republic of China.
(3)Chang-Hua Breeding Animal Propagation Station, Taiwan Livestock Research
Institute, Chang-Hua, Taiwan, Republic of China.
(4)Council of Agriculture, Executive Yuan, Taipei, Taiwan, Republic of China.
¤¤°ê¯bªª¾Ç·|·|»x ²Ä25¨÷ ²Ä2´Á 139¡ã148, 1996